The Polymorphism of FecXG Region Exon 2 of BMP15 Gene in Hisari Sheep

نویسندگان

  • Elham Rezvannejad Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran
  • Sajad Bahrami Department of Medical Nanotechnology, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran
  • Seyed Azim Mousavizadeh Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
  • Yadollah Bahrami Young Researchers Club and Elites, Khorasgan Branch, Islamic Azad University, Khorasgan, Iran
چکیده مقاله:

Booroola gene is one of the major genes in the enhancement of ovulation rate and could be an attractive candidate gene for ovulation rate in sheep. Molecular technologies have been widely used for discovery of mutations in animal species. These mutations have major effects on the important economic traits of sheep and goats. This study was conducted to identify the mutation in FecXG region of exon 2 of BMP15 gene which is associated with twining using polymerase chain reaction-restriction fragment length polymorphism method in Hisari Sheep. For this purpose, blood samples were obtained from 110 sheep. DNA was extracted using the salting out method and quantity and quality properties of DNA were determined using electrophoresis gel. Afterward, a 141 bp fragment, which contains the given polymorphic site of BMP15 gene, was amplified by the specific primers. Restriction enzyme (HinfI G/ANTC) cuts the "wild-type" allele and as a result of digestion, 112 and 29 bp fragments produced. Mutant type allele is not cut because of the removal of the intended cutting site. All the samples showed the similar monomorphic of genotype (+/+) (wild genotype). Results of this study indicate that the investigated polymorphism is not present in the hisari sheep

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

the study of aaag repeat polymorphism in promoter of errg gene and its association with the risk of breast cancer in isfahan region

چکیده: سرطان پستان دومین عامل مرگ مرتبط با سرطان در خانم ها است. از آنجا که سرطان پستان یک تومور وابسته به هورمون است، می تواند توسط وضعیت هورمون های استروئیدی شامل استروژن و پروژسترون تنظیم شود. استروژن نقش مهمی در توسعه و پیشرفت سرطان پستان ایفا می کند و تاثیر خود را روی بیان ژن های هدف از طریق گیرنده های استروژن اعمال می کند. اما گروه دیگری از گیرنده های هسته ای به نام گیرنده های مرتبط به ا...

15 صفحه اول

Exon 2 Ovar-DRB1 gene polymorphism in the Iranian Sangsari sheep

The major histocompatibility complex (MHC) plays a central role in the control of disease resistance and immune response. Extensive genetic diversity in MHC genes provides a valuable source for genetic improvement, via selection, in many domestic animals. Exon 2 of the class II MHC, termed Ovar-DRB1 in domestic sheep (Ovis aries), has been suggested as important disease resistance and immune re...

متن کامل

the effect of e-64 on the developmental competence of sheep cocs during in vitro maturation

in the present study, the effect of e-64 at different concentrations (0.5, 1, 5 and 10 µm) added to (1) the ivm medium on oocyte nuclear maturation and developmental competence of ovine oocytes, and (2) to the ivc medium on embryonic development of ovine embryos were investigated.

Polymorphism of sheep POU1F1 gene exon 6 and 3'UTR region and their association with milk production traits

The purpose of the study was to detect the AluI and DdeI polymorphisms within POU1F1 gene exon 6 and 3'UTR region in Turkish sheep breeds, and their association with milk productive traits. Jugular blood samples were collected from 50 Sakiz, 50 White Karaman, and 50 Awassi sheep using EDTA as an anticoagulant. PCR-RFLP and sequencing analysis were performed to investigate possible polymorphisms...

متن کامل

: the effect of sericin levels (silk glue protein) on rate of in vitro maturation, fertilization and culture of sheep oocytes

هدف از آزمایش اول بررسی اثر سطوح مختلف سریسین [0 (control), 0.1, 0.5, 1.0, 2.5 %] افزوده شده به محیط , ivm بر cumulus cell expansion، بلوغ هسته و توسعه متوالی جنین، در گوسفندان نژاد سنجابی در فصل تولید مثلی می باشد. از سرگیری میوز به وسیله خارج شدن اولین پولار بادی اندازه گیری و هم چنین درصد رسیدن جنین های دو سلولی به مرحله کلیواژ و بلاستوسیت نیز به عنوان نشانه ای از میزان شایستگی توسعه اولیه ج...

polymorphism in exon2 of bmp15 gene in iranian sangsari sheep

fertility rate is an economically important trait in sheep, which is influenced by genetic and environment. so far, three genes have been identified that affects this trait, one of them would be the bmp family, the most famous one is bmp15. different mutations in the bmp15 gene, increases reproductive performance and growth rate in sheep. the aim of this study was to investigate the genetic and...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ذخیره در منابع من قبلا به منابع من ذحیره شده

{@ msg_add @}


عنوان ژورنال

دوره 4  شماره 1

صفحات  7- 13

تاریخ انتشار 2018-03-01

با دنبال کردن یک ژورنال هنگامی که شماره جدید این ژورنال منتشر می شود به شما از طریق ایمیل اطلاع داده می شود.

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023